Primer3
Output
PRIMER
PICKING RESULTS: Arabidopsis thaliana, 3-methyladenine DNA glycosylase
No
mispriming library specified
Using
1-based sequence positions
OLIGO start len
tm gc% any
3' seq
LEFT PRIMER 713
20 60.01 50.00
4.00 2.00 GGATCTTGTCGGATTCTGGA
RIGHT
PRIMER 910 20
59.89 50.00 4.00
0.00 GAGGCAAATCCTCCATACCA
SEQUENCE
SIZE: 1287
INCLUDED
REGION SIZE: 1287
PRODUCT
SIZE: 198, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00
1
ATGGGTGAACATTCACCTTCTCAACCATCATCTCATACTCTTCCTCCAAACCAACCGGAA
61
TCACCCAATCACGAAACCCCTAATCCAATTCCACCGGAAACCAACGATGACGACTCCGCC
121
TCATCCGCCGGAGTTTCTGGCTCAATCGTCTCATCAACCACCATTGAAGCACCACAAGTC
181
ACTGAATTAGGCAATGTATCGTCTCCACCTACCAAGATCCCTCTCCGTCCTCGAAAAATC
241
AGAAAACTTTCACCCGACGACGACGCTTCCGACGGATTCAATCCGGAGCATAACCTTTCT
301
CAGATGACGACGACGAAACCGGCAACGAAGAGTAAATTATCACAGTCACGCACTGTAACC
361
GTGCCGAGAATCCAAGCAAGGTCTCTCACTTGTGAAGGCGAGCTTGAAGCAGCTCTTCAC
421
CATCTCCGAAGTGTTGATCCTCTGCTCGCGTCTTTAATCGACATTCATCCACCACCAACG
481
TTCGAGACTTTTCAGACTCCGTTCTTGGCGTTGATTCGAAGCATTCTTTATCAGCAATTA
541
GCAGCTAAAGCAGGTAATTCAATCTACACACGCTTCGTTGCCTTATGCGGCGGTGAAAAC
601
GGCGTCGTGCCAGAGAATGTATTGCCTTTAACACCTCAACAGCTTCGTCAAATTGGTGTT
661 TCCGGGCGTAAAGCGAGTTACCTTCATGATCTTGCTAGGAAATATCAAAATGGGATCTTG
>> >>>>> >
721
TCGGATTCTGGAATAGTGAATATGGATGAGAAATCTTTGTTCACGATGTTAACAATGGTG
>>> >> >>>>> >>
781
AATGGGATTGGGTCATGGTCGGTCCATATGTTTATGATAAACTCTCTTCATAGACCTGAT
841
GTGTTACCAGTTAATGATCTTGGTGTAAGGAAGGGTGTGCAGATGCTTAATGGTATGGAG
<<<< <<< << <
901
GATTTGCCTCGTCCATCCAAAATGGAGCAGCTTTGCGAGAAATGGCGTCCGTATAGATCA
<< <<<< << <<
961 GTAGCGTCTTGGTACTTGTGGCGGCTCATTGAATCTAAAAATACACCACCTAATGCTGCG
1021
GCTGCTACGGCCGGAGCTGCTCTTTCGTTTCCACAGTTGGAAGACATTCAGCAACAAGAA
1081 CAAGAACAGCAACATCAACAACATCAGCAACAACAGCCGCAGCTTATGGACCCTCTTAAT
1141
AATGTGTTCAGTATCGGGATGTTCCAGGATAGCATCTATGTAGGGAATTGGTTTGAGGTT
1201 GATTTCAATGGCTGGATAGACCTATTTGATCTTCTGATTTTAGTTGATAGATTACTCATG
1261 TCCAGTCCTTTGCTTATCTTAATTTAG
KEYS (in
order of precedence):
>>>>>>
left primer
<<<<<<
right primer
ADDITIONAL
OLIGOS
start len tm
gc% any 3'
seq
1 LEFT PRIMER 283 20 60.21
50.00 4.00 1.00 CCGGAGCATAACCTTTCTCA
RIGHT PRIMER 482 20 60.10
45.00 6.00 2.00 AACGTTGGTGGTGGATGAAT
PRODUCT SIZE: 200, PAIR ANY COMPL: 4.00,
PAIR 3' COMPL: 1.00
2 LEFT PRIMER 137 20 59.94
50.00 2.00 2.00 CTGGCTCAATCGTCTCATCA
RIGHT PRIMER 335 20 59.75
45.00 4.00 0.00 TTACTCTTCGTTGCCGGTTT
PRODUCT SIZE: 199, PAIR ANY COMPL: 3.00,
PAIR 3' COMPL: 1.00
3 LEFT PRIMER 137 20 59.94
50.00 2.00 2.00 CTGGCTCAATCGTCTCATCA
RIGHT PRIMER 334 20 60.25
50.00 4.00 0.00 TACTCTTCGTTGCCGGTTTC
PRODUCT SIZE: 198, PAIR ANY COMPL: 3.00, PAIR
3' COMPL: 0.00
4 LEFT PRIMER 98 20 59.97
50.00 4.00 1.00 AAACCAACGATGACGACTCC
RIGHT PRIMER 297 20 59.53
45.00 6.00 1.00 AAGGTTATGCTCCGGATTGA
PRODUCT SIZE: 200, PAIR ANY COMPL: 4.00,
PAIR 3' COMPL: 1.00
Statistics
con too in in no
tm tm high high
high
sid many tar excl
bad GC too
too any 3' poly end
ered Ns get reg
GC% clamp low
high compl compl
X stab ok
Left 10646
0 0 0
3 0 3117 4656 27 94 0 162 2587
Right 10674
0 0 0
5 0 3428
4419 2 12 0 163 2645
Pair Stats:
considered
2615, unacceptable product size 1575, high any compl 10, high end compl 59, ok
971
primer3 release 0.9
(primer3_www_results.cgi v 0.2)