Primer3
Output
PRIMER
PICKING RESULTS Rattus norvegicus,
3-methyladenine-DNA glycosylase
No
mispriming library specified
Using
1-based sequence positions
OLIGO start len
tm gc% any
3' seq
LEFT
PRIMER 175 20
60.08 55.00 4.00
1.00 ACAGTCAGAGCAGCAGCAGA
RIGHT
PRIMER 374 20
59.68 50.00 4.00
2.00 CACGGAGTTCTGTTCCATCA
SEQUENCE
SIZE: 963
INCLUDED
REGION SIZE: 963
PRODUCT
SIZE: 200, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00
1
CTCTAAAGAACCGGTGTCCGTGGTGCTCCCCGACGCCGAGCACCCTGCATTTCCTGGACG
61
AACCCGCCGCCCAGGGAATGCCAGAGCAGGATCCCAAGTGACCGGATCTCGTGAGGTCGG
121
CCAGATGCCAGCGCCGCTTTCACGAAAGATCGGGCAAAAAAAGCAGCAACTGGCACAGTC
> >> >>>
181
AGAGCAGCAGCAGACCCCTAAGGAGAAACTCTCTTCGACCCCTGGCCTCCTGCGAAGTAT
>> >> >> >> >>
241
CTACTTCTCGAGCCCAGAGGACCGCCCTGCCCGGCTAGGACCGGAGTATTTTGACCAGCC
301
CGCAGTCACCCTAGCCCGTGCATTTCTGGGACAGGTCCTTGTCCGGCGACTTGCTGATGG
<< << <<
361
AACAGAACTCCGTGGGCGCATTGTAGAGACTGAGGCATACTTGGGGCCAGAAGACGAAGC
<< << << < <<< << <<
421
TGCCCACTCAAGGGGTGGCCGGCAGACCCCCCGTAACCGTGGCATGTTCATGAAACCTGG
481
AACTCTGTACGTGTACCTCATCTATGGCATGTACTTCTGCTTGAATGTCTCCAGTCAAGG
541
GGCTGGGGCTTGTGTCTTGCTAAGAGCACTAGAGCCATTGGAGGGCCTAGAGACCATGCG
601
GCAGCTTCGAAACTCCCTCCGGAAAAGCACTGTCGGCCGTTCCCTCAAGGACCGCGAGCT
661
CTGTAATGGTCCCTCCAAACTGTGCCAGGCCCTAGCCAGGAGTAAGAGCTTTGACCAGCG
721
AGACCTGGCCCAAGATGAGGCTGTGTGTGGCTGGAGCATGGCCCTCTGGAGTCCAGCAGC
781
CAGCTGTGGTGGCGGCAGCCGCAGGTATTGGCCATGCAGGGGAATGGACACAGAAGCCCT
841
TGCGCTTCTATGTCCAGGGCAGCCCATGGGTCAGTGTGGTAGATAGAGTGGCTGAACAGA
901
TGTATCAGCCTCAGCAAACAGCGTGCTCTGATTGTTCAAAAGTAAAATAAATAACTTGTT
961 TGT
KEYS (in
order of precedence):
>>>>>> left primer
<<<<<<
right primer
ADDITIONAL
OLIGOS
start len tm
gc% any 3'
seq
1 LEFT PRIMER 176 20 60.08
60.00 4.00 3.00 CAGTCAGAGCAGCAGCAGAC
RIGHT PRIMER 374 20 59.68
50.00 4.00 2.00 CACGGAGTTCTGTTCCATCA
PRODUCT SIZE: 199, PAIR ANY COMPL: 4.00,
PAIR 3' COMPL: 1.00
2 LEFT PRIMER 175 20 60.08
55.00 4.00 1.00 ACAGTCAGAGCAGCAGCAGA
RIGHT PRIMER 366 20 59.98
50.00 4.00 2.00 TCTGTTCCATCAGCAAGTCG
PRODUCT SIZE: 192, PAIR ANY COMPL: 4.00,
PAIR 3' COMPL: 1.00
3 LEFT PRIMER 176 20 60.08
60.00 4.00 3.00 CAGTCAGAGCAGCAGCAGAC
RIGHT PRIMER 366 20 59.98
50.00 4.00 2.00 TCTGTTCCATCAGCAAGTCG
PRODUCT SIZE: 191, PAIR ANY COMPL: 4.00,
PAIR 3' COMPL: 2.00
4 LEFT PRIMER 176 20 60.08
60.00 4.00 3.00 CAGTCAGAGCAGCAGCAGAC
RIGHT PRIMER 375 20 60.51
55.00 4.00 0.00 CCACGGAGTTCTGTTCCATC
PRODUCT SIZE: 200, PAIR ANY COMPL: 4.00,
PAIR 3' COMPL: 2.00
Statistics
con too in in no
tm tm high
high high
sid many tar excl
bad GC too too any 3' poly end
ered Ns get reg
GC% clamp low
high compl compl
X stab ok
Left 6971
0 0 0 52 0 721 5036 0 22 43 168 929
Right 6972
0 0 0 181 0 946 4559 1 4 44 166 1071
Pair Stats:
considered
428, unacceptable product size 302, high end compl 2, ok 124
primer3 release 0.9